DNA

Part:BBa_K5017003:Design

Designed by: Christos Kalogeropoulos   Group: iGEM23_Patras-Med   (2023-10-11)


CD8a Hinge domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because there was no experience in previous plasmid design, after studying a variety of design guides, we followed a specific design plasmid design strategy. In this strategy, the sequences of the desired genes that make up the receptor were found from NCBI database and then, the exact sequence of the parts of these genes that have been used in plasmids that have been constructed and deposited into Addgene repository, were searched using the BLAST global alignment tool. By this method we were led in a more secure way to the total sequence of the plasmid.


Source

This sequence was synthesized by Genscript and cloned into pGenLenti vector backbone.

References